@phdthesis {saracoglu2001,
	title = {Bildanalyse von M-FISH},
	year = {2001},
	publisher = {IWR, Fakult{\"a}t f{\"u}r Physik und Astronomie, Univ.\ Heidelberg},
	abstract = {Multiplex-FISH is a combinatorial staining technique that allows the simultaneous detection and discrimination of all human chromosomes. Using at least five fluorochromes all chromosomes can be uniquely labeled in a combinatorial way and identified by their specific spectral signature. Within this thesis I developed a novel approach for the automated analysis of M-FISH images, yielding robust classification results and allowing the analysis of M-FISH images of different experiments. The method combines spectral information with spatial information to tesselate the image into regions of similar color. Subsequently a cluster analysis in color space and a final classification step are performed to identify the biological targets. This approach is applicable to images of different M-FISH experiments, allowing the analysis of interchromosomal as well as intrachromosomal abnormalities in the genome. It also allows the 3D analysis of M-FISH labeled chromosomes in interphase nuclei.},
	url = {http://www.ub.uni-heidelberg.de/archiv/1805/},
	author = {Saracoglu, K.}
}
