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[(tions)-574.2(have)-566.3(been)-570.3(published,)-574.8(and)-568.6(transfer)-569.2(of)-572.3(the)-572.8(available)]TJ
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[(methods)-299.3(to)-300.4(other)-298.1(projects)-295.3(is)-302(sometimes)-295.2(difcult)-299.2(due)-295.1(to)-300.4(miss-)]TJ
T*
[(ing)-283.2(standardization)-282.9(and)-284.1(strong)-284.9(restrictions)-282.9(on)-284.4(input)-282(formats,)]TJ
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[(software)-506.3(tool)-502.7(deployment,)-506.1(and)-499.1(so)-503.6(on.)-502.3(Nevertheless,)-507.1(these)]TJ
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[(solutions)-260.7(have)-256.5(an)-255.8(enormous)-262.7(potential)-261.5(to)-256.2(support)-262.2(the)-256.7(analysis)]TJ
T*
[(of)-268.8(current)-277.7(and)-265.1(future)-276.1(image-based)-272.8(phenotypic)-272.8(studies)-270.1(and)-271.5(to)]TJ
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[(To)-252.4(promote)-252.1(a)-252.4(better)-252.4(integration)-256(of)-256.2(image)-251.5(analysis)-250.5(software)]TJ
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[(with)-299.9(microscopy)-297.5(imaging)-300.9(strategies,)-299(in)-297.8(particular)-300.8(in)-291.5(the)-300.9(con-)]TJ
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[(text)-344.4(of)-338.4(large-scale)-347.2(screening)-344.6(projects,)-342.5(it)-339.1(is)-340(crucial)-342.3(to)-338.4(compre-)]TJ
T*
[(hensively)-222.4(survey)-217.1(existing)-221(image)-213.6(analysis)-218.9(solutions)-216.5(and)-220.9(to)-211.9(rate)]TJ
T*
[(their)-185(potential)-185.6(for)-187.3(application)-182.6(to)-186.6(other)-184.3(problems.)-185.2(Furthermore,)]TJ
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[(one)-246.3(needs)-254.1(a)-239.8(better)-252.4(understanding)-248.7(of)-249.8(the)-250.3(requirements)-247.1(of)-249.8(both)]TJ
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[(biologists)-559.2(and)-556(computer)-553.5(scientists)-564.6(in)-550.7(image)-555(analysis)-560.3(that)]TJ
T*
[(should)-306.2(then)-304.3(guide)-303.1(how)-298.6(precisely)-311.1(data)-299.7(are)-303.6(acquired)-302.6(and)-303.1(ana-)]TJ
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[(quality)-289.1(that)-282(is)-289.4(necessary)-287.9(for)-282.2(image)-283.1(analysis,)-291.3(or)-281.7(set)-286.2(acceptable)]TJ
T*
[(error)-238.7(margins)-231.7(for)-231.6(biological)-236(feature)-236.2(detection.)-243.3(Clarifying)-232.3(such)]TJ
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T*
[(long-term)-386.3(goal)-389.6(would)-385.3(be)-383.7(the)-389.5(design)-388.6(of)-382.6(standardized)-389.9(work-)]TJ
T*
[(ows)-337.5(for)-339.1(data)-337.6(acquisition,)-342.2(data)-337.6(handling,)-343.2(image)-333.7(processing,)]TJ
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[(and)-537(data)-533.6(analysis,)-531.6(integrating)-542.2(many)-529.7(existing)-537.2(generic)-535.9(and)]TJ
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T*
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T*
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[(re)10.8(q)-6.2(u)-3.2(irement)10.8(s)-439.9(f)10.8(o)-5.1(r)-432.5(a)10.8(u)-9(t)10.8(o)-11.4(m)10.8(a)-5.2(t)-2.3(ic)-424.8(image)-425.4(p)-3.2(rocessing)-429.9(o)1.7(f)-426(z)-1.4(e)10.8(b)-6.2(ra)10.8()-10.7(s)3.2(h)]TJ
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T*
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T*
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[(tivities)-306.9(in)-308.9(bio)-4.8(l).7(ogica)10.6(l)-314.9(imaging)-310(i).7(s)-306.9(g)-.1(ive)10.6(n)-11.7(,)-308.5(f)10.6(o)-11.6(llowed)-308.5(b)-.1(y)-310(a)10.6(n)-321.1(o)1.5(ver-)]TJ
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T*
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[(physiolo)-5(gy)10.4(,)-357.3(p)-3.6(harmacology,)-352.9(a)10.4(n)-11.5(d)-353(t)10.4(o)-5.5(x)-4.4(icology)-354.4(i).5(n)-353.4(A)2.7(pplicat)10.4(i)-6.2(o)-5(ns)]TJ
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27.9978 74.5598 TD
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[(w)-5.1(e)-623(hav)-4.2(e)-623(Dis)-4.8(c)-2.7(us)-4.8(sion)-6.8(:)-619.7(o)-6.2(pen)-626.5(q)9.2(u)-9.2(e)-3.3(st)-3.9(ions)-624.4(and)-626.1(f)9.2(u)-11.6(t)-3.9(ur)-4.1(e)-623(t)-3.9(rends)]TJ
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0 Tc
[(Requirements)-341.2(for)-330.9(Automated)-336.5(Processing)]TJ
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[(Automated)-430.1(image)-422.2(analysis)-427.5(is)-428.5(not)-423.9(limited)-428.8(to)-426.9(any)-424.6(type)-423.4(of)]TJ
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T*
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T*
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T*
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[(t)-386.2(data)-375.5(from)-380.9(multiphoton)-387.3(or)-382.8(SPIM)-380.1(microscopy)-379.7(or)-382.9(high-)]TJ
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[(processing)-258.2(of)-249.8(imaging)-244(data)-249.1(are)-253(not)-246.9(conned)-255(to)-249.8(any)-247.6(particular)]TJ
T*
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T*
[(therefore,)-479(focus)-473.1(on)-467.7(the)-471.7(requirements)-474.7(of)-471.1(the)-471.6(community)-471.9(to)]TJ
T*
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T*
[(light)-230.3(or)-224.8(temperature)-229.5(pulses\),)-227.1(automated)-232.2(microscopy)-228(imaging,)]TJ
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[(data)-381.9(management,)-387.9(image)-378(processing,)-387.6(and)-379(evaluation)-381.4(of)-382.6(the)]TJ
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[(results)-286.8(\(Fig.)-281.3(1\).)]TJ
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[(cells,)-465.7(embryos,)-462.9(larvae,)-462.1(adult)-463.1(sh,)-459.5(drug-specic)-466.5(phenotypic)]TJ
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[(effects)-438.1(at)-429.9(the)-433.7(cellular)-435(or)-433.4(subcellular)-435.8(level,)-434.2(and)-429.5(mutants)-434(ex-)]TJ
T*
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[(typical)-284(readouts)-287.2(for)-282.2(such)-277.2(questions)-290.3(could)-281.8(be)-276.2(as)-279(follows:)]TJ
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()Tj
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8.9663 0 0 8.9663 331.4834 292.8755 Tm
[(place)-338.7(preference,)-337.2(swimming)-335.4(speed,)]TJ
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7.9702 0 0 7.9702 323.0361 283.2377 Tm
()Tj
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8.9663 0 0 8.9663 331.4834 281.9338 Tm
[(detection)-341.5(of)-332(embryonic)-338.4(lethality,)]TJ
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7.9702 0 0 7.9702 323.0361 272.296 Tm
()Tj
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8.9663 0 0 8.9663 331.4834 270.9921 Tm
[(morphological)-341.3(changes)-333.7(in)-335.7(embryos/larvae,)]TJ
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7.9702 0 0 7.9702 323.0361 261.2975 Tm
()Tj
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8.9663 0 0 8.9663 331.4834 259.9936 Tm
[(changes)-302.1(in)-297.8(reporter)-297.3(gene)-298.5(expression)-300.9(in)-297.8(embryos/larvae,)]TJ
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7.9702 0 0 7.9702 323.0361 250.3558 Tm
()Tj
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8.9663 0 0 8.9663 331.4834 249.0519 Tm
[(the)-338.9(number)-336.1(of)-332(labeled)-336.3(cells)-336.3(in)-335.7(specic)-336.5(tissues,)-334.6(and)]TJ
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7.9702 0 0 7.9702 323.0361 239.4141 Tm
()Tj
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8.9663 0 0 8.9663 331.4834 238.1102 Tm
[(movement)-337.4(of)-338.4(cells)-336.4(and)-328.4(tissues)-338(in)-335.7(3D)]TJ
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[(t)-335.6(imaging.)]TJ
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[(Given)-518.5(this)-514.5(diversity)-517.1(of)-515.4(assays)-519.5(and)-511.7(readouts,)-517.8(the)-515.9(corre-)]TJ
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[(sponding)-383.6(requirements)-386.2(in)-380(terms)-385.6(of)-376.3(specimen)-385.6(manipulation,)]TJ
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[(imaging,)-190(data)-192.2(management,)-191.9(and)-189.3(image)-188.3(processing)-195(are)-183.5(equally)]TJ
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[(imaging)-250.3(on)-240.1(either)-248.2(xed)-248.3(or)-243.7(live)-241.6(specimens,)-250.7(from)-241.8(one-cell)-253(stage)]TJ
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T*
[(whole)-668.8(organism)-664.5(interacting)-669.8(with)-666.6(its)-669.2(environment.)-669.9(Once)]TJ
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[(the)-307.3(context)-305.6(of)-300.4(the)-307.3(experiment)-306(has)-301.4(been)-304.8(chosen,)-303.8(the)-307.3(design)-300.1(of)]TJ
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[(the)-440(imaging)-440(protocol)-443.6(needs)-437.5(to)-439.5(be)-434.3(adapted)-443(to)-433.2(the)-440(expected)]TJ
T*
[(results)-286.8(and)-277.8(to)-281.5(the)-288.3(requirements)-285(for)-282.2(image)-276.8(processing.)]TJ
1.1128 -1.2203 TD
[(The)-303.5(spatiotemporal)-311.9(resolution)-310.6(of)-300.4(the)-307.3(acquired)-308.9(images,)-300(the)]TJ
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