In-situ microscopy for on-line characterization of cell-populations in bioreactors, including concentration measurements by depth from focus

TitleIn-situ microscopy for on-line characterization of cell-populations in bioreactors, including concentration measurements by depth from focus
Publication TypeJournal Article
Year of Publication1995
AuthorsSuhr, H, Wehnert, G, Schneider, K, Bittner, C, Scholz, T, Geißler, P, Jähne, B, Scheper, T
JournalBiotechnology and Bioengineering
Volume47
Pagination106--116
Abstract

A new technique is presented which allows the use of a front-end sensor head for in situ and on-line characterization of cell concentration and cell size during fermentation. An epifluorescence microscope is mounted in a port of a bioreactor viewing directly into the agitated broth. Still images from cells are generated using pulsed illumination. They are directly visualized on a monitor and used for automatic image analysis. The cell concentration and morphological information are determined by counting and evaluating the cell images with respect to their depth from focus characteristic. An in situ microscope was successfully tested during yeast fermentations and yielded results which correlated well with results from a hemocytometer.

DOI10.1002/bit.260470113
Citation Keysuhr1995a